Open AccessANTIBODY-DEPENDENT CELL-MEDIATED CYTOTOXICITY TESTS FOR IMMUNOSUPPRESSION MONITORING OF TRANSPLANT PATIENTS
Author(s) -
Mutsuo Sasaki,
Paul I. Terasaki,
Howard Silberman,
Thomas V. Berne
Publication year - 1979
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/00007890-197904000-00008
Subject(s) - immunosuppression , antibody dependent cell mediated cytotoxicity , dialysis , cytotoxicity , antibody , medicine , kidney transplant , kidney , immunology , whole blood , kidney transplantation , biology , monoclonal antibody , in vitro , biochemistry
The antibody-dependent cell-mediated cytotoxicity (ADCC) effector activity and phytohemagglutinin (PHA) response of kidney transplant patients were monitored by a new whole blood microassay that permitted performance of 20 tests using only 1 ml of blood. Eleven patients were followed on as many as 55 different occasions with these tests. The ADCC activity of dialysis patients was essentially the same (78% chromium release as compared with 83% in normal controls). However, patients with kidney transplants undergoing immunosuppression had approximately one-half the chromium release (40%). Daily fluctuation in the chromium release could not be used as a predictor of subsequent rejection. The PHA response was as much as 50-fold less in the immunosuppressed patients. Both values also tended to be slightly higher among patients who were doing well than those who were doing poorly. This may reflect the need for greater immunosuppression in the rejecting patients.