Open AccessMLR-R AND MLR-S GENE PRODUCTS ARE EXPRESSED ON DIFFERENT LYMPHOID CELLS
Author(s) -
J Brochier
Publication year - 1977
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/00007890-197707000-00009
Subject(s) - thymidine , lymphocyte , antigen , microbiology and biotechnology , mixed lymphocyte reaction , t lymphocyte , biology , immunology , ficoll , complement receptor , chemistry , t cell , complement system , immune system , biochemistry , peripheral blood mononuclear cell , in vitro
The role of human B and T lymphocytes in the mixed lymphocyte reaction (MLR) was investigated using T- and B-enriched populations obtained either by centrifugation of E rosetting cells over a Ficoll-Isopaque gradient or after B or T cells had been killed by specific antihuman T lymphocyte antigen or antihuman B lymphocyte and monocyte antigen sera, respectively, in the presence of complement. Purified T lymphocytes responded to allogenic cells whereas they were unable to stimulate the MLR; in contrast, purified B lymphocytes were not activated but were found to be good stimulators. Treatment of the MLR, at the time of addition of tritiated thymidine, by antihuman T lymphocyte antigen serum and complement completely suppressed the thymidine incorporation, indicating that the bulk of the proliferative response was supported by the T lymphocytes. Additional experiments with populations depleted of complement or Fc receptor-bearing lymphocytes did not suggest that these B lymphocyte subpopulations played any major role in the MLR.