Open AccessAN IN VITRO ASSAY OF CELL-MEDIATED CYTOTOXICITY
Author(s) -
Bilha Schechter,
Michaël Feldman
Publication year - 1976
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/00007890-197610000-00004
Subject(s) - cytotoxicity , in vitro , cell , chemistry , microbiology and biotechnology , biology , biochemistry
A method is described for measuring cell-mediated cytotoxicity, based on the incorporation of labeled leucine into actively synthesized proteins in viable target cells which have survived interaction with effector lymphocytes. The method was studied with in vitro or in vivo sensitized lymphocytes in xenogeneic or allogeneic systems. This method was found to be applicable to quantitative determination of cell-mediated cytotoxicity by in vitro sensitized lymphocytes against a syngeneic tumor.