Open AccessTHE USE OF VIABLE FROZEN LYMPHOCYTES FOR STUDIES IN HUMAN TUMOR IMMUNOLOGY
Author(s) -
Sidney H. Golub,
Hector L. Sulit,
Donald L. Morton
Publication year - 1975
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/00007890-197503000-00001
Subject(s) - immune system , cytotoxic t cell , immunology , melanoma , peripheral blood , frozen section procedure , medicine , lymphatic system , pathology , biology , cancer research , in vitro , biochemistry
Viable frozen lymphocytes displayed activity in blastogenesis assays that was indistinguishable from freshly prepared lymphoid cells. Similarly, cytotoxic activity of lymphocytes against melanoma target cells from melanoma patients was only slightly affected by the freezing procedure. Frozen lymphocytes provided a highly reproducible source of cells in these assays. The use of viable frozen peripheral blood lymphoid cells for the retrospective analysis of a cancer patient's immune response is described.