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Expression of Hypoxia Inducible Factor 1 α and Von Hippel Lindau Protein in Human Middle Ear Cholesteatoma
Author(s) -
Adunka Oliver,
Gstoettner Wolfgang,
Knecht Rainald,
Kierner Antonius C.
Publication year - 2003
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1097/00005537-200307000-00018
Subject(s) - cholesteatoma , hypoxia (environmental) , middle ear , biology , medicine , anatomy , chemistry , ophthalmology , organic chemistry , oxygen
Objective To evaluate the role of hypoxia in cholesteatoma progression. Study Design Immunohistochemical analysis of paraffin‐embedded human specimens. Methods Thirteen middle ear cholesteatomas and 10 samples of normal human external ear canal skin were stained immunohistochemically for the presence of hypoxia inducible factor 1 α (HIF 1 α) and Von Hippel Lindau protein. Specimens were then analyzed semiquantitatively. Results Staining for both antibodies could be detected in all cholesteatomas (perimatrix and matrix), as well as in the samples of normal human ear canal skin. Cholesteatoma specimens showed statistically significant increased staining when compared with normal human skin and mucosa. The age of patients and relapse surgery affected immunohistochemical staining of HIF 1 α and Von Hippel Lindau protein. Conclusions Elevated staining intensities for HIF 1 α and Von Hippel Lindau protein in cholesteatoma tissue indicated that cholesteatoma may be hypoxic. In addition, our data indicated that relapse cholesteatomas yield a higher degree of hypoxia than ears without surgery.