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Single Cell Analysis of Adenoid CD5 + B Cells and Their Protective Contributions to Nasopharyngeal Immunity
Author(s) -
Arita Miori,
Kodama Satoru,
Suzuki Masashi,
Mogi Goro
Publication year - 2003
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1097/00005537-200303000-00017
Subject(s) - cd5 , adenoid , antibody , b cell , biology , flow cytometry , immunology , microbiology and biotechnology
Objectives CD5 + B cells are phenotypically and functionally distinct from the conventional (CD5 − ) B cells, and the function of CD5 + B cells in the upper respiratory tract remains unknown. A previous study showed that immunoglobulin A–producing cells in the adenoid play a protective role in the nasopharynx. In the present study, the contribution of adenoid CD5 + B cells to nasopharyngeal immunity at the single cell level was investigated. Study Design In vitro laboratory study. Methods Mononuclear cells were isolated from adenoids of children aged 1 to 12 years, and the frequency of CD5 + B cells was determined by flow cytometry. The numbers of cells producing immunoglobulin M, immunoglobulin G, and immunoglobulin A in sorted adenoid CD5 + B cells were determined by enzyme‐linked immunospot assay. Further, to characterize adenoid CD5 + B cells, the expression of various surface molecules was analyzed by flow cytometry. Results The results showed that adenoids of young children contain a relatively large number of CD5 + B cells, which have a greater capacity for antibody production than do CD5 − B cells. CD5 + B cells also differed from CD5 − B cells in the expression of interleukin receptors Il‐4R, IL‐5R, and IL‐10R as well as CD27, B7‐1, B7‐2, Fas, and Bcl‐2. Conclusions These findings suggest that adenoid CD5 + B cells contribute to protective immunity by forming a first line of defense in the upper respiratory tract of young children and that they are probably regulated in a manner that differs from that of CD5 − B cells.