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Effects of Dexamethasone on Mucin Gene Expression in Cultured Human Nasal Epithelial Cells
Author(s) -
Ishinaga Hajime,
Takeuchi Kazuhiko,
Kishioka Chikako,
Yagawa Mayuko,
Majima Yuichi
Publication year - 2002
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1097/00005537-200208000-00020
Subject(s) - mucin , gene expression , glucocorticoid , dexamethasone , messenger rna , lipopolysaccharide , microbiology and biotechnology , cell culture , stimulation , biology , gene , chemistry , endocrinology , biochemistry , genetics
Objective To clarify 1) which mucin gene expression is influenced by glucocorticoid and 2) whether glucocorticoid influences steady‐state mucin expression or mucin gene expression induced by lipopolysaccharide. Methods Dissociated cells obtained from nasal polys were cultured on a collagen gel substrate in an air‐liquid interface. Dexamethasone was added to the culture medium in the steady‐state and prior to the stimulation by LPS. RNAs were extracted from culture cells, and semiquantitative reverse transcriptase‐polymerase chain reaction was performed for MUC2, MUC5AC, MUC5B, MUC8, and β‐actin. Results Dexamethasone did not influence steady‐state messenger RNA levels of either mucin gene. Dexamethasone suppressed lipopolysaccharide‐induced messenger RNA levels of MUC8. Conclusion Glucocorticoid does not influence steady‐state mucin gene expression but suppresses mucin gene expression induced by secretagogues such as lipopolysaccharide.