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Tissue‐Engineered Cartilage on Biodegradable Macroporous Scaffolds: Cell Shape and Phenotypic Expression
Author(s) -
Baek ChungHwan,
Lee JongChan,
Jung YongGi,
Ko YeJeung,
Yoon Jun Jin,
Park Tae Gwan
Publication year - 2002
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1097/00005537-200206000-00021
Subject(s) - plga , chondrocyte , cartilage , chemistry , microbiology and biotechnology , in vitro , immunostaining , phenotype , type ii collagen , immunohistochemistry , anatomy , biochemistry , biology , immunology , gene
Abstract Objective The purpose of the study was to establish in vitro culture of chondrocytes on biodegradable, poly(D,L‐lactic‐co‐glycolic acid) [PLGA] scaffolds. Study Design Laboratory experiment using cartilage of rat rib and biodegradable scaffolds. Methods Chondrocytes were cultured on a poly‐hydroxyethyl methacrylate (poly‐HEMA)–coated dish, proliferated, and transferred into the PLGA scaffolds. Phenotypic expression of cells was examined according to the condition of poly‐HEMA coating. Morphological, biochemical, and immunohistochemical characteristics of cells cultured within PLGA scaffolds were also examined. Results Chondrocytes cultured on a poly‐HEMA–coated dish aggregated into distinct nodules containing large clusters of spherical cells and showed cartilage‐specific phenotype, collagen type II. The results of immunostaining and reverse transcriptase–polymerase chain reaction of cells cultured within PLGA scaffolds showed cartilage‐specific morphological appearance and structural characteristics such as lacunae and expression of collagen type II. Conclusion The chondrocytes cultured on a poly‐HEMA–coated dish and PLGA scaffolds showed chondrocyte‐specific phenotypes and morphological appearance.