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Application of Unbiased Stereology on Archival Human Temporal Bone
Author(s) -
Tang Yong,
Lopez Ivan,
Ishiyama Akira
Publication year - 2002
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1097/00005537-200203000-00022
Subject(s) - stereology , spiral ganglion , scarpa's ganglion , vestibular system , spiral (railway) , temporal bone , anatomy , biology , vestibular nerve , neuroscience , mathematics , inner ear , mathematical analysis , endocrinology
Objective The current report presents unbiased stereological sampling and counting strategies for estimating the total number of neurons in the spiral and vestibular ganglia of the archival human temporal bone. Study Design Application of unbiased stereology using archival human temporal specimens. Observational study. Methods The practical sampling and counting strategies for estimating the number of neurons are presented. To illustrate the method described, the total number of neurons in spiral and vestibular ganglia from archival human temporal bone was estimated. Results The study found, on average, 41,643 (coefficient of variation = 0.12) spiral ganglion neurons in the subjects with an average age of 35.6 years (age range, 22–60 y) and, on average, 27,635 (coefficient of variation = 0.10) vestibular ganglion neurons in the subjects with an average age of 28.6 years (age range, 2–58 y). Conclusions The total number of primary auditory and vestibular ganglion neurons from the current study differs significantly from the previously published data using assumption‐based methods. The unbiased stereological technique presented in the current report thus would be invaluable for future studies on such specimens, including the study of the changes of neurons during aging and various pathological conditions.