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Repair of an Osseous Facial Critical‐Size Defect Using Augmented Fibrin Sealant
Author(s) -
Tholpady Sunil S.,
Schlosser Rodney,
Spotnitz William,
Ogle Roy C.,
Lindsey William H.
Publication year - 1999
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1097/00005537-199910000-00007
Subject(s) - sealant , fibrin , medicine , fibrin tissue adhesive , dentistry , surgery , materials science , immunology , composite material
Objective: Osseous defects of the head and neck are a common challenge for the otolaryngologist. To develop improved reconstructive options, osteoconductive engineering experiments are being conducted. A nasal critical‐size defect (CSD) model has previously been described in which less than 7% bone healing is observed over 6 months. An implant containing fibrin sealant with and without osteoprogenitor cells is evaluated in this model. Study Design: Randomized controlled trial using a rodent model. Methods: A nasal CSD was surgically created in 18 male retired breeder Sprague‐Dawley rats. Six animals were not implanted with any material, six received fibrin sealant consisting of fibrin (25 mg/mL) and thrombin (1000 U/mL), and six were implanted with fibrin sealant and rat calvarial osteoprogenitor cells (1.8 × 10 6 cells/mL). Thirty days later, the animals were examined at necropsy by planimetry, histological analysis of new bone growth, and radiodensitometric analysis of bone thickness. Results: A thin layer of bone covered the defect in all of the treated animals. A statistically significant increase in bone density ( P < .05) between fibrin sealant plus osteoprogenitor cells and each of the other groups was shown using radiodensitometric analysis. Histological analysis also confirmed this difference. Conclusion: Osteoprogenitor cells contained within fibrin sealant result in a greater augmentation of bone regeneration than controls or fibrin sealant alone.