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Mutational Analyses of Candidate Genes in Human Squamous Cell Carcinomas
Author(s) -
Petroianu Andy,
Boson Wolfanga L.,
Bale Allen E.,
Friedman Eitan,
De Marco Luiz
Publication year - 1999
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1097/00005537-199904000-00027
Subject(s) - carcinogenesis , biology , gene , single strand conformation polymorphism , cancer research , cell , candidate gene , population , esophagus , polymerase chain reaction , genetics , medicine , environmental health , anatomy
Objectives and Study Design: Squamous cell carcinomas are common malignancies and a major cause of mortality. The molecular mechanisms involved in tumorigenesis remain largely unknown, but sequence alterations have been identified in coding regions of several genes. Primary squamous cell carcinomas of various tissues (skin, head and neck, esophagus, lung, penis, uterus, and vagina) from 52 patients were analyzed for the presence of mutations within several candidate genes presumably involved in tumorigenesis: Gsα, Gi 2 α, GTPase activating protein (GAP), and patched ( PTCH ) genes. Methods: Mutational analysis scheme included polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE), single stranded conformational polymorphism (SSCP), and selected sequence analysis. Results and Conclusion: No tumor had any evidence of mutations in any of these analyzed genes. Mutations within these genes do not occur frequently in an unselected population of patients with squamous cell carcinomas.