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Recognition of a Dominant Epitope in Bovine Heat‐Shock Protein 70 in Inner Ear Disease
Author(s) -
Bloch Donald B.,
Gutierrez Jesus A.,
Guerriero Vince,
Rauch Steven D.,
Bloch Kurt J.
Publication year - 1999
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1097/00005537-199904000-00019
Subject(s) - epitope , hsp70 , pathogenesis , antigen , antibody , heat shock protein , meniere's disease , blot , inner ear , recombinant dna , medicine , epitope mapping , biology , immunology , disease , microbiology and biotechnology , pathology , biochemistry , anatomy , gene
Abstract Objective: To investigate the specificity of anti‐bodies to heat‐shock protein 70 (HSP70) in patients with idiopathic, progressive, bilateral sensorineural hearing loss (IPBSNHL) and Meniere disease. Study Design: Test immunoreactivity of patients' sera using recombinant human (rh) and bovine (rb) HSP70, as well as segments representing different regions of bovine HSP70 as antigen. Methods: Sera were tested by Western blotting. Results: Of 52 patients with IPB‐SNHL, 40 sera reacted only with rbHSP70; 12 reacted with both rbHSP70 and rhHSP70. Sera from 13 patients with IPBSNHL and from 8 with Meniere disease were tested on the panel of bovine HSP70 segments. Eleven and 7 samples, respectively, reacted with amino acid segment 427‐461 from the carboxy (C)‐terminal region of the molecule. Conclusion: In IPBSNHL and Meniere disease, antibodies are directed primarily against an epitope(s) within the C‐terminal region of HSP70 where diversity in sequence among different species, including possible pathogens, is greatest. These findings may provide clues to the pathogenesis or specific serodiagnosis (or both) of diseases of the inner ear.