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Th‐2 type cytokine receptors in allergic rhinitis and in response to topical steroids
Author(s) -
Wright Erin D.,
Christodoulopoulos Pota,
Small Peter,
Frenkiel Saul,
Hamid Qutayba
Publication year - 1998
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1097/00005537-199810000-00019
Subject(s) - cytokine , receptor , immunology , medicine , allergy
Objectives: Th‐2 type cytokine production (inter‐leukin‐4 [IL‐4] and interleukin‐5 [IL‐5]) has been demonstrated to play a significant role in the pathophysiology of allergic rhinitis (AR), and the treatment of AR with topical corticosteroids has been shown to reduce the expression of Th‐2 type cytokines in vivo. However, the contribution and expression of Th‐2 type cytokine receptors in AR and their response to corticosteroid treatment remain to be clarified. Objectives of the current study are 1. To examine the expression of the cytokine IL‐4 and IL‐5 receptors (IL‐4R and IL‐5R) in a nasal allergen challenge model and to contrast this with the expression of the receptor for the Th‐1 type cytokine, interferon‐gamma receptor (IFN‐γR), and 2. to examine the effects of pretreatment with topical corticosteroid before allergen challenge on the expression of these same receptors. Study Design: Randomized prospective study involving 14 ragweed‐allergic subjects evenly divided between placebo and corticosteroid pretreatment. Methods: Immunocytochemistry (alkaline phosphatase‐antial‐kaline phosphatase labeling [APAAP] technique) was used to stain nasal biopsy specimens before and after allergen challenge. Antibodies used included anti‐CD3, CD4, CD8, MBP, IL‐4R, IL‐5R, and IFN‐γR. Results: Following allergen challenge, we observed a significant increase in the Th‐2 type cytokine receptors (IL‐4R and IL‐5R; P < .05), as well as a significant decrease in the expression of the Th‐1 type cytokine receptor (IFN‐γR; P < .05). Pretreatment with topical corticosteroids before nasal allergen challenge resulted in decreased expression of IL‐4R ( P < .05) and IL‐5R ( P < .05) and increased expression of IFN‐γR ( P < .05). Further, IL‐4R and IL‐5R expression correlated with eosinophil infiltration in the tissues. Conclusions: We have demonstrated that in AR, cytokine receptors for IL‐4, IL‐5, and IFN‐γ follow a similar pattern to their ligands. In addition, pretreatment with topical corticosteroids was shown to alter the cytokine receptor expression pattern from a Th‐2 profile more toward a Th‐1 profile. Laryngoscope , 108:1528–1533,1998