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Changes in Immunostaining of Inner Ears After Antigen Challenge Into the Scala Tympani
Author(s) -
Ichimiya Issei,
Kurono Yuichi,
Hirano Takashi,
Mogi Goro
Publication year - 1998
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1097/00005537-199804000-00023
Subject(s) - spiral ligament , cochlea , labyrinthitis , inner ear , anatomy , fibrocyte , keyhole limpet hemocyanin , immunostaining , albumin , venule , pathology , medicine , biology , immune system , immunology , organ of corti , immunohistochemistry , microcirculation , hearing loss , audiology
To study the mechanisms of immune responses and immune injuries in inner ears, labyrinthitis was induced by inoculation of keyhole limpet hemocyanin (KLH) into the scala tympani of systemically sensitized guinea pigs. Inner ears were then immunostained for KLH, immunoglobulin G (IgG), albumin, connexin26 (Cx26), and sodium‐potassium adenosine triphosphate (Na,K‐ATPase). Inflammatory cells containing KLH were observed in the scala tympani and in the collecting venule of the spiral modiolar vein (SMV). Spiral ligament, spiral limbus, and blood vessels including the SMV were diffusely positive for IgG and albumin. Immunoreactivity for Cx26 and Na,K‐ATPase was decreased compared with the normal ears in the fibrocytes of the spiral ligament. These results suggest that inflammatory cells and blood constituents could extravasate into the cochlea from blood vessels and that fibrocyte damage in the spiral ligament could cause cochlear dysfunction.