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Effects of Phenylephrine on Ciliary Beat in Human Nasal Respiratory Epithelium: Quantitative Measurement by Video‐Computerized Analysis
Author(s) -
Min YangGi,
Yun YeongSeok,
Rhee ChaeSeo,
Sung MyungWhun,
Lee KangSoo,
Ju Myung Sil,
Park KwangSuk
Publication year - 1998
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1097/00005537-199803000-00019
Subject(s) - phenylephrine , incubation , anesthesia , medicine , repeated measures design , respiratory system , analysis of variance , mucous membrane of nose , incubation period , chemistry , endocrinology , anatomy , blood pressure , biochemistry , mathematics , statistics
The in vitro effects of phenylephrine solution on ciliary beat frequency (CBF) in terms of different concentrations and exposure times were investigated using a video‐computerized analysis technique. Nasal epithelial cells were taken from inferior turbinate of 10 volunteers by scraping the nasal mucosa with a cytology brush. CBF was measured in five different concentrations including 0.125%, 0.25%, 0.5%, 1%, and 2.5%. Each specimen was incubated in different solution for 6 days and CBF was measured at 30 minutes, 1 hour, 2 hours, 4 hours, 8 hours, 12 hours, 1 day, 2 days, 3 days, 4 days, 5 days, and 6 days. CBF decreased significantly after 12‐hour incubation in 0.125% phenylephrine solution and after 8‐hour incubation in 0.25% phenylephrine solution, both clinically used concentrations. There were significant decreases in CBF after incubation in 0.5% phenylephrine for 2 hours, in 1% for 1 hour, and in 2.5% for 30 minutes ( P < 0.05, repeated measure analysis of variance [ANOVA]). CBF of the nasal respiratory ciliated cells significantly decreased with increasing concentrations of phenylephrine solution and with increasing incubation times at the same concentration ( P < 0.05, repeated measure ANOVA). The results of this study suggest that phenylephrine may inhibit ciliary beat in vitro by its pharmacological effect at lower concentrations than clinically used ones.