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Effects of Chronic Administration of Kanamycin on the Basement Membrane Anionic Sites in the Crista Ampullaris of Guinea Pigs
Author(s) -
Suzuki Mitsuya,
Yamasoba Tatsuya,
Kaga Kimitaka
Publication year - 1998
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1097/00005537-199801000-00015
Subject(s) - kanamycin , basement membrane , guinea pig , chemistry , cell , inner ear , cationic polymerization , anatomy , microbiology and biotechnology , medicine , endocrinology , biology , biochemistry , gene , organic chemistry
The authors investigated the effects of chronic kanamycin (KM) administration on the basement membrane (BM) anionic sites in the ampulla by studying the binding of cationic polyethyleneimine (PEI). KM sulfate was administered intramuscularly 'to guinea pigs with normal Preyer's reflexes daily for 10 or 17 days. The PEI distribution was unchanged on the subepithelial BM in the dark cell region and on the capillary BM in the crista ampullaris. However, PEI binding decreased significantly on the subepithelial BM in the sensory cell and transitional cell regions of those guinea pigs administered KM for 17 days. In the sensory cell region, the PEI distribution did not recover until 6 weeks after KM treatment. Findings suggest that chronic administration of KM severely alters the number of subepithelial BM anionic sites in the sensory cell region.