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Parathyroid Visualization With Cibacron Blue/BB5‐G1
Author(s) -
King Raymund C.,
Mills Stanley L.,
Medina Jesus E.
Publication year - 1996
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1097/00005537-199611000-00011
Subject(s) - conjugate , monoclonal antibody , visualization , chemistry , medicine , antibody , pathology , computer science , mathematics , artificial intelligence , immunology , mathematical analysis
BB5‐G1 is a murine monoclonal antibody with proven affinity for human parathyroid tissue. The authors conjugated BB5‐G1 to cibacron blue, a watersoluble dye, in an attempt to enhance intraoperative parathyroid visualization. Athymic nude mice possessing intramuscularly implanted human parathyroid tissue were infused with the conjugate, then surgically explored. Intraoperative findings were video‐captured, then analyzed by three independent observers and by computer, generating subjective and objective data quantifying parathyroid visualization. Parathyroid tissue implants were stained and were easily visualized. The implants in the test mice displayed higher computer‐generated color intensity ratings (mean, 10.25 units) compared to control mice (mean, 6.10 units) ( p <0.5). Histologic sections confirmed the presence of dye and antibody within parathyroid tissue. This represents the first study to use such a conjugate to selectively enhance parathyroid visualization.