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Heterogeneity of Squamous Cell Carcinomas of the Head and Neck‐Analysis of Tumor Biologic Factors and Proliferation Rates
Author(s) -
Jacob Roland,
Welkoborsky Hans J.,
Mann Wolf J.,
Höfken Frank,
Dienes Hans P.,
Freije James E.
Publication year - 1996
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1097/00005537-199609000-00023
Subject(s) - proliferating cell nuclear antigen , grading (engineering) , pathology , immunohistochemistry , ki 67 , proliferation marker , biopsy , cell growth , biology , head and neck squamous cell carcinoma , cell , head and neck cancer , medicine , cancer , ecology , genetics
Specimens obtained from five different tumor regions in 12 patients who underwent surgery for squamous cell carcinoma (SCC) of the oropharynx were examined. The evaluation of each biopsy included quantitative DNA measurements based on image analysis, immunohistochemical assessment of proliferations markers (i.e., Ki67‐MIB1, proliferating cell nuclear antigen [PCNA]), and morphological tumorfront grading. From single cell measurements, several DNA indices were derived which are known to reflect tumor aneuploidy. The results revealed a marked variation of proliferation and cellular differentiation in different regions of tumors and a wide intraindividual variation between particular tumors for all markers examined. There was good correlation between DNA data and proliferative cell fractions (Ki67 score, PCNA score). With the use of diagrams, three‐dimensional distribution of proliferation rates and markers reflecting tumor aggressiveness within each tumor was obtained. The results confirmed previous clinical and histological observations that SCCs of the oropharynx are heterogeneous tumors. One might expect that the regions with increased proliferation and aggressiveness may predict the location of possible tumor recurrence.