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Immunobiological Peculiarities of Cholesteatoma in Children: Quantification of Epithelial Proliferation by MIB 1
Author(s) -
Bujía Jesús,
Holly Anja,
AntolíCandela Francisco,
Tapia Mario Guzman,
Kastenbauer Ernst
Publication year - 1996
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1097/00005537-199607000-00015
Subject(s) - cholesteatoma , immunohistochemistry , pathology , monoclonal antibody , medicine , significant difference , middle ear , alkaline phosphatase , antibody , biology , anatomy , immunology , surgery , enzyme , biochemistry
Cholesteatoma in children is characterized by a more extensive and rapid growth in the middle ear and mastoid cavities. The growth characteristics of the cholesteatoma in 20 children were studied using the monoclonal antibody MIB 1, which recognizes a nuclear antigen expressed by cells in the G1, S, and G2/M phases. Specimens of normal adult auditory meatal skin (n = 15) and adult cholesteatoma (n = 15) served as controls. The tissue specimens were prepared for immunohistochemical examination using the alkaline phosphatase‐antialkaline phosphatase method and an automatic image analyzer. Specimens of normal skin revealed an average MIB 1 score of 9.2 ± 3.1%. Child and adult cholesteatomas showed higher values. The average MIB 1 score was higher in child cholesteatoma (42 ± 9.4%) than in adult cholesteatoma (28.2± 6%). This difference was statistically significant ( P <.01). Our results confirm a significant increase of the proliferative rate of cholesteatoma keratinocytes in children, giving an explanation for the more aggressive clinical behavior observed in these patients. Laryngoscope, 106:865‐868, 1996