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Quantitative Analysis of Interleukin‐1‐Alpha Gene Expression in Middle Ear Cholesteatoma
Author(s) -
Bujía J.,
Kim C.,
Boyle D.,
Hammer C.,
Firestein G.,
Kastenbauer E.
Publication year - 1996
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1097/00005537-199602000-00021
Subject(s) - cholesteatoma , in situ hybridization , epithelium , middle ear , biology , bone resorption , alpha (finance) , stroma , messenger rna , pathology , immunohistochemistry , inner ear , microbiology and biotechnology , anatomy , immunology , gene , medicine , endocrinology , biochemistry , construct validity , nursing , radiology , patient satisfaction
Regardless of its origin, cholesteatoma is characterized by the presence of a keratinizing epithelium with an hyperproliferative behavior leading to a very important bone resorption. Previous studies have demonstrated overexpression of interleukin‐1 (IL‐1) protein in middle ear cholesteatoma by immunohistochemistry and enzyme‐linked immunosorbent assay, suggesting a significant role for IL‐1‐alpha. In this study, the presence of IL‐1‐alpha messenger ribonucleic acid (mRNA) was quantified by in situ hybridization on frozen sections (n=10) and by computer‐assisted image analysis. Human skin obtained from the external ear canal (n=10) was used as the control. A higher percentage of cells hybridized for the antisense probes IL‐1‐alpha mRNA was found in cholesteatoma epithelium. Furthermore, keratinocytes of the suprabasal cell layers were also found to contain specific hybridizations. Some cells in cholesteatoma stroma also contained IL‐1‐alpha mRNA transcripts. The results of this study confirm the central role of IL‐1‐alpha in the epithelium hyperproliferation and bone resorption observed in middle ear cholesteatoma.