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Guinea Pig Inner Ear Antigens
Author(s) -
Cao M. Y.,
Deggouj N.,
Gersdorff M.,
Tomasi J.P.
Publication year - 1996
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1097/00005537-199602000-00019
Subject(s) - guinea pig , inner ear , antigen , western blot , biology , microbiology and biotechnology , anatomy , immunology , biochemistry , endocrinology , gene
In this study, the authors attempted to develop a method of extracting guinea pig inner ear antigens for otoimmunological research, and to investigate the distribution of the antigens in the various structures of the inner ear. The antigens were extracted either from the entire or from various parts of the guinea pig inner ear. These antigens were separated on sodium dodecylsulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) gels. Western blot techniques were nsed to test sera from patients with inner ear disease against guinea pig inner ear protein extraets. It was found that the various molecular weight antigens in the inner ear were associated with the different structures of the inner ear. The sera of 37.5% N=80) of patients reacted with two bands (30 and 58 kd) of the guinea pig inner ear immunoblots. The 58‐kd band was not specific to the inner ear, but instead was also found in the immunoblots of other guinea pig tissues (brain, lung, and liver). This study suggests that the various antigens of interest could be further extracted and purified from the corresponding locations of the inner ear.