Open AccessSecretion of Endothelin-1 and Adrenomedullin by SW-13 Human Adrenocortical Carcinoma Cells
Author(s) -
Kazuhiro Takahashi,
Ayako Yoshinoya,
Osamu Murakami,
Kazuhito Totsune,
Shigeki Shibahara
Publication year - 2000
Publication title -
journal of cardiovascular pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.762
H-Index - 100
eISSN - 1533-4023
pISSN - 0160-2446
DOI - 10.1097/00005344-200036051-00114
Subject(s) - adrenomedullin , adrenocortical carcinoma , vasoactive intestinal peptide , endocrinology , medicine , secretion , endothelin receptor , endothelin 1 , northern blot , calcitonin , endothelins , radioimmunoassay , calcitonin gene related peptide , biology , peptide hormone , adrenal medulla , neuropeptide , hormone , gene expression , gene , receptor , catecholamine , biochemistry
The adrenal medulla and pheochromocytomas are known to secrete various neuropeptides and vasoactive peptides. On the other hand, the production and secretion of peptides by adrenocortical tumors have not been studied in detail. The study reported here therefore set out to examine these two functions for two vasoactive peptides, endothelin-1 (ET-1) and adrenomedullin (ADM) in SW-13 human adrenocortical carcinoma cells by radioimmunoassay and Northern blot analysis. Both immunoreactive ET (irET) and irADM were detected in the culture medium of SW-13 cells. Northern blot analysis showed the expression of ET-1 and ADM mRNAs in SW-13 cells. On the other hand, no significant amounts of calcironin-gene-related peptide, corricotropin-releasing-hormone, neuropeptide Y or urocorlin were secreted by SW-13 cells. This study has shown that ET-1 and ADM are the two unique vasoactive peptides that are produced and secreted by adrenocortical carcinoma cells.