Open AccessExpression of Endothelin-1 and Endothelin Receptors in Cultured Human Glioblastoma Cells
Author(s) -
Masahiko Sone,
Kazuhiro Takahashi,
Kazuhito Totsune,
Osamu Murakami,
Zenei Arihara,
Fumitoshi Satoh,
Toraichi Mouri,
Shigeki Shibahara
Publication year - 2000
Publication title -
journal of cardiovascular pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.762
H-Index - 100
eISSN - 1533-4023
pISSN - 0160-2446
DOI - 10.1097/00005344-200036051-00113
Subject(s) - endothelin receptor , northern blot , cell culture , endothelin 1 , microbiology and biotechnology , receptor , radioimmunoassay , secretion , biology , western blot , messenger rna , reverse transcription polymerase chain reaction , reverse transcriptase , endocrinology , polymerase chain reaction , gene , biochemistry , genetics
Production and secretion of endothelin-1 (ET-1) by a human glioblastoma cell line, T98G, were studied by radioimmunoassay and Northern blot analysis. Immunoreactive ET was detected in the culture medium of T98G (17.6 +/- 0.6 fmol/10(5) cells/24 h, mean +/- SEM, n = 5). Reverse-phase high-performance liquid chromatography (HPLC) of immunoreactive ET in the culture medium extract showed a single peak eluting in the position of ET-1. Northern blot analysis showed expression of ET-1 mRNA in T98G cells. Treatment with interferon-gamma decreased the expression of ET-1. Treatment with TNFalpha or interleukin-1beta (IL-1beta) increased the expression of ET-1. Furthermore, reverse transcriptase polymerase chain reaction (RT-PCR) showed expression of endothelin-A- and -B- (ET(A) and ET(B)) receptor mRNAs in T98G glioblastoma cells. These findings indicate that glioblastoma cells produce and secrete ET-1, and express ET receptor mRNAs. ET-1 secreted by glioblastoma cells may act locally on tumor cells, possibly as a growth modulator.