Open AccessThe Endothelin-2/Vasoactive Intestinal Contractor Gene
Author(s) -
Kaname Saida,
Tsuyoshi Uchide,
Akemi Usui,
XiaoDong Gao,
Noboru Tomizuka,
Shinichi Oka,
Hiromi Masuda
Publication year - 2000
Publication title -
journal of cardiovascular pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.762
H-Index - 100
eISSN - 1533-4023
pISSN - 0160-2446
DOI - 10.1097/00005344-200036051-00005
Subject(s) - vasoactive intestinal peptide , vasoactive , transfection , microbiology and biotechnology , gene , reporter gene , endothelin receptor , alkaline phosphatase , biology , gene expression , reverse transcription polymerase chain reaction , reverse transcriptase , polymerase chain reaction , chemistry , enzyme , endocrinology , genetics , biochemistry , neuropeptide , receptor
In order to understand the physiological roles of vasoactive intestinal contractor (VIC)/endothelin-2 (ET-2), we examined the expression of this peptide by specific reverse transcriptase polymerase chain reaction (RT-PCR) analysis and found that PC12 rat pheochromocytoma cells express the VIC gene. The 5'-flanking 1.0 kilo base pair (kb) region of the mouse VIC gene is sufficient to express a secreted alkaline phosphatase (SEAP) reporter gene in transiently transfected PC12 cells. The 1.0 kb promoter region may contain cis-acting elements that determine the rate of the VIC gene transcription in PC12 cells.