Open AccessEndothelin-1 Stimulates Hypertrophy and Contractility of Neonatal Rat Cardiac Myocytes in a Serum-Free Medium. II
Author(s) -
Takahiko Suzuki,
Hiroyuki Hoshi,
Hajime Sasaki,
Youji Mitsui
Publication year - 1991
Publication title -
journal of cardiovascular pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.762
H-Index - 100
eISSN - 1533-4023
pISSN - 0160-2446
DOI - 10.1097/00005344-199100177-00052
Subject(s) - nicardipine , contractility , contraction (grammar) , medicine , myocyte , endocrinology , endothelin 1 , muscle hypertrophy , chemistry , protein kinase c , biology , kinase , calcium , biochemistry , receptor
The effect of endothelin-1 (ET-1) on rat cardiac myocytes cultured in a serum-free medium was determined. Cardiac myocytes cultured in our medium showed an increased rate of protein synthesis, morphological size, contraction rate, and Ca2+ uptake when ET-1 was added. These actions of ET-1 were inhibited by a protein kinase C inhibitor, H-7. Among these four phenomena, contraction and Ca2+ uptake were inhibited by a Ca2+ channel blocker, nicardipine. Therefore, it is likely that the signal for ET-1 to induce these phenomena is transduced by kinase C, and Ca2+ uptake is related to maintaining a high contraction rate.