Open AccessSerotonin Release and Phosphoinositide Breakdown in Thrombin-Induced Activation of Human Platelets
Author(s) -
P. Marché,
Francine Rendu,
Jacques Maclouf,
A Girard,
S Lévy-Toledano
Publication year - 1985
Publication title -
journal of cardiovascular pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.762
H-Index - 100
eISSN - 1533-4023
pISSN - 0160-2446
DOI - 10.1097/00005344-198500077-00005
Subject(s) - thrombin , platelet , serotonin , phosphatidylinositol , phospholipase c , chemistry , platelet activation , inositol , pi , secretion , phospholipase , biochemistry , endocrinology , medicine , biology , enzyme , receptor , signal transduction
The release reaction and the metabolism of inositol lipids were studied in parallel in washed human platelets following the activation by low doses of thrombin. The breakdown of phosphatidylinositol 4,5-bisphosphate (PI-P2) was accompanied by the release of serotonin. These events preceded the breakdown of the other phosphoinositides and the release of alpha-granules and lysosome constituents, respectively. The secretion of serotonin probably is triggered by products of thrombin-induced activation of the phospholipase C directed against PI-P2.