Open AccessA Dot Immunobinding Assay to Detect Anti-α-gliadin Antibodies in Celiac Disease
Author(s) -
Manuela Bittolo,
Tarcisio Not,
Sandra Perticarari,
Sabina Cauci,
G. Graziosi,
Roberto Marzari
Publication year - 1990
Publication title -
journal of pediatric gastroenterology and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 131
eISSN - 1536-4801
pISSN - 0277-2116
DOI - 10.1097/00005176-199010000-00009
Subject(s) - gliadin , antibody , medicine , coeliac disease , nitrocellulose , whole blood , immunology , incubation , microbiology and biotechnology , gluten , disease , pathology , biochemistry , chemistry , biology , membrane
A dot immunobinding assay to detect anti-alpha-gliadin-specific antibodies in the sera or whole blood of enteropathic patients is described here. The method is based on the adsorption of alpha-gliadin as a spot onto nitrocellulose sheets. After incubation with the patient sample, the detection of specific antibodies is performed with alkaline phosphatase-conjugated goat anti-human (IgA or IgG) antibodies. Twenty-one celiac serum samples together with 18 enteropathic or disease controls and 44 healthy controls were analyzed. The classical ELISA test and the dot test gave comparable results. The dot test gave reliable result even when whole blood was tested. The method proved to be simple and sensitive.