Open AccessEffect of Different Particles on Cell Proliferation in Polymer Scaffolds Using a Solvent-Casting and Particulate Leaching Technique
Author(s) -
Sungho Suh,
Ji Youn Shin,
Jin Hoon Kim,
Young Tae Kim,
Chung Hwan Beak,
Dong Ik Kim,
Hojoong Kim,
Seong Soo Jeon,
In Wook Choo
Publication year - 2002
Publication title -
asaio journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.961
H-Index - 66
eISSN - 1538-943X
pISSN - 1058-2916
DOI - 10.1097/00002480-200209000-00003
Subject(s) - gelatin , scaffold , biocompatibility , plga , leaching (pedology) , materials science , solvent , chemical engineering , polymer , porosity , chemistry , biomedical engineering , composite material , nanotechnology , nanoparticle , metallurgy , organic chemistry , medicine , environmental science , engineering , soil science , soil water
Solvent-casting and particulate leaching are widely used in the manufacturing of porous polymer scaffolds. Salt is the most commonly used particulate because it is easily available and very easy to handle. Gelatin particles are another candidate for this method because they are known as a material that enhances cell attachment and proliferation. In this study, we compared the biocompatibility of the two scaffolds made from either salt (salt scaffold) or gelatin particles (gelatin scaffold). Sieved particles of salt and gelatin (particle size ranging 100-180 um) were dispersed in a poly-lactic-co-glycolic acid (PLGA)/chloroform solution and cast in a Teflon container. The solvent was allowed to evaporate and residual amounts were removed by vacuum drying. The particles were allowed to leach out by immersion in warm water (40 degrees C). Cultured chondrocytes (from knee cartilage) and smooth muscle cells (from bladder) were seeded on each scaffolds (5 x 10(6)/cm2) and cultured for 3 weeks, and their proliferation was compared using hematoxylin and eosin staining. These results demonstrated that the gelatin scaffold showed better attachment of cells at the initial stage, and both cell types showed much better proliferation of cells during 3 months. The better performance of a gelatin scaffold also contributed to the better connection of pores at the same porosity.