Open AccessEffects of Plasma on Adhesion of Biofilm Forming Pseudomonas aeruginosa and Staphylococcus epidermidis to Fibrin Substrate
Author(s) -
Benson De,
Burns Gl,
Mohammad Sf
Publication year - 1996
Publication title -
asaio journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.961
H-Index - 66
eISSN - 1538-943X
pISSN - 1058-2916
DOI - 10.1097/00002480-199609000-00069
Subject(s) - staphylococcus epidermidis , biofilm , microbiology and biotechnology , pseudomonas aeruginosa , fibrin , chemistry , adhesion , coagulase , bacteria , staphylococcus aureus , staphylococcus , biology , immunology , organic chemistry , genetics
Bacterial adhesion has been identified as the critical initial step in the pathogenesis of foreign body related infection. Recent investigations have shown microbial binding to implanted polymeric materials using specific adhesion of bacteria to immobilized plasma proteins, such as fibrin. These proteins are though to function as bridging molecules to facilitate bacterial colonization of the surface. The authors' results indicated a significant reduction in adhesion of biofilm forming Pseudomonas aeruginosa and coagulase negative Staphylococcus epidermidis to immobilized fibrin strands in the presence of platelet poor plasma (PPP) as compared to studies performed with phosphate buffered saline and Hank's balanced salt solution. A 10-fold decrease in the number of adherent bacteria was noted for samples exposed to PPP as compared to control samples. The effective range of PPP concentrations capable of producing the marked decrease in binding to fibrin strands was determined to be 1-100% for P. aeruginosa and 4-100% for S. epidermidis.