Open AccessExtracorporeal Hybridization of Proximal Renal Tubular Cells and an Artificial Membrane for the Purpose of Beta2Microglobulin Removal
Author(s) -
Tsutomu Sanaka,
Tetuzou Agishi,
Teruyuki Hayashi,
Yoriko Hayasaka,
Mayumi Yasuo,
Kana Fukui,
Ota K,
Noriko Sugino
Publication year - 1989
Publication title -
asaio transactions
Language(s) - English
Resource type - Journals
eISSN - 2375-0952
pISSN - 0889-7190
DOI - 10.1097/00002480-198907000-00114
Subject(s) - microgram , cell culture , chemistry , microbiology and biotechnology , kidney , membrane , chromatography , biology , biochemistry , endocrinology , in vitro , genetics
The present study was designed to develop a bioartificial kidney hybridized with LLC-PK1 cells for the purpose of beta 2microglobulin (B2M) removal. Cells were prepared in polystyrene culture wells; the bottom of each was covered with collagen fiber and the cells grown as monolayer cultures. Each well was inoculated with 1 ml cell suspensions with 0.0, 1.0 X 10(6), 2.0 X 10(6), or 4.0 X 10(6) cells/well. B2M was added to the culture system at a concentration of 80 micrograms/dl. Results were as follows: 1) Concentration of B2M in the supernatant of 4 X 10(6) cultivated LLC-PK1 cells was decreased from 79.32 +/- 2.41 micrograms/dl to 67.02 +/- 3.00 micrograms/dl (n = 6, p less than 0.01) at 2 hr; 2) Concentration of B2M in the supernatant of 1 X 10(6), 2 X 10(6), and 4 X 10(6) cultivated LLC-PK1 cells was 75.06 +/- 2.31 micrograms/dl (n = 6, p less than 0.05); 67.27 +/- 11.41 micrograms/dl (n = 6, p less than 0.001); and 67.02 +/- 3.00 micrograms/dl (n = 6, p less than 0.001), respectively, vs. 79.32 +/- 2.41 micrograms/dl in controls at 2 hr; 3) There was a significant positive correlation between the efficacy of B2M removal and cell number; and 4) These results suggested that it is possible to develop an artificial kidney hybridized with proximal tubular cells that possesses a high affinity for B2M removal.