Open AccessDevelopment of a Novel Biocompatibility Test Method Based on Intracellular Calcium Ion Mobilization
Author(s) -
Yoshiaki Takemoto,
Takehisa Matsuda,
Eiichi Ozeki,
T. Kishimoto,
Mamiko Maekawa,
T Akutsu
Publication year - 1989
Publication title -
asaio transactions
Language(s) - English
Resource type - Journals
eISSN - 2375-0952
pISSN - 0889-7190
DOI - 10.1097/00002480-198907000-00048
Subject(s) - polyacrylonitrile , biocompatibility , membrane , chemistry , calcium , platelet activation , copolymer , calcium in biology , cellulose acetate , cellulose , platelet , intracellular , ethylene glycol , elution , biophysics , transmembrane protein , chromatography , polymer chemistry , chemical engineering , biochemistry , organic chemistry , receptor , engineering , immunology , biology , polymer
This paper presents a novel evaluation method for determining platelet/material interaction at the transmembrane level by incorporation of a Ca-sensitive fluorophore (Fura-2) into platelets. The transmembrane stimulation of platelets which are eluted from commercially available artificial dialyzers was quantitatively measured as the elevation of intracellular free calcium ion concentration. This method clearly differentiated platelet response to hydrophilic (ethylene vinyl alcohol copolymer and cellulose) vs. hydrophobic (polyacrylonitrile and cellulose acetate) membranes, and offers a versatile means to understanding platelet/material interaction.