Open AccessCross-clade HIV-1 neutralization by an antibody fragment from a lupus phage display library
Author(s) -
Sangeeta Karle,
Stephanie Planque,
Yasuhiro Nishiyama,
Hiroaki Taguchi,
Yong Zhou,
María Salas,
Douglas F. Lake,
Perumal Thiagarajan,
Frank C. Arnett,
Carl V. Hanson,
Sudhir Paul
Publication year - 2004
Publication title -
aids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.195
H-Index - 216
eISSN - 1473-5571
pISSN - 0269-9370
DOI - 10.1097/00002030-200401230-00026
Subject(s) - antibody , phage display , neutralization , virology , systemic lupus erythematosus , clade , human immunodeficiency virus (hiv) , biology , fragment (logic) , repertoire , neutralizing antibody , microbiology and biotechnology , virus , gene , immunology , genetics , medicine , phylogenetics , disease , pathology , physics , computer science , acoustics , programming language
A single-chain fragment containing antibody V domains (scFv) isolated from a lupus antibody library displayed the ability to bind gp120 and the conserved gp120 determinant composed of residues 421-436. The scFv neutralized R5 and X4-dependent HIV-1 strains from clades B, C, and D. The lupus repertoire may be useful as a source of neutralizing antibodies to HIV.