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A recombinant vaccinia virus based ELISPOT assay detects high frequencies of Pol-specific CD8 T cells in HIV-1-positive individuals
Author(s) -
Marie Larsson,
Xia Jin,
Bharat Ramratnam,
Graham Ogg,
Jose Engelmayer,
Marie-Ange Demoitié,
Andrew J. McMichael,
William I. Cox,
Ralph M. Steinman,
Douglas F. Nixon,
Nina Bhardwaj
Publication year - 1999
Publication title -
aids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.195
H-Index - 216
eISSN - 1473-5571
pISSN - 0269-9370
DOI - 10.1097/00002030-199905070-00005
Subject(s) - elispot , virology , vaccinia , antigen , biology , hiv antigens , cd8 , orthopoxvirus , microbiology and biotechnology , biotinylation , virus , recombinant dna , immunology , viral disease , gene , biochemistry
HIV-1-specific CD8 T cells are considered to be critical in anti-HIV responses. It is important to quantify these cells and to determine their antigenic targets. Here quantification of interferon (IFN)-gamma secreting, virus-specific cells was achieved with an enzyme linked immuno spot (ELISPOT) assay.

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