Open AccessRapid Analysis of Carcinoembryonic Antigen Levels in Gallbladder Bile Identification of Patients at High Risk of Colorectal Liver Metastasis
Author(s) -
Timothy J. Yeatman,
Arthur K. Kimura,
Edward M. Copeland,
Kirby I. Bland
Publication year - 1991
Publication title -
annals of surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.153
H-Index - 309
eISSN - 1528-1140
pISSN - 0003-4932
DOI - 10.1097/00000658-199102000-00003
Subject(s) - carcinoembryonic antigen , medicine , radioimmunoassay , gallbladder , gastroenterology , cancer
Recently it was found that immunoanalysis of carcinoembryonic antigen (CEA) levels in gallbladder bile may be a sensitive method to detect colorectal liver metastases in humans. Methods used in the past for the detection of CEA in various body fluids were cumbersome and time consuming, requiring acid extraction, extensive dialysis, and column purification. Single-step, solid-phase radioimmunoassays, designed specifically for serum CEA analysis, were developed commercially to replace these methods. Parameters and methodology necessary to adapt these kits for Parameters and methodology necessary to adapt these kits for use with gallbladder bile are presented here. A combination of pretreatment procedures for bile, before radioimmunoassay, permit rapid, reproducible, and accurate measurement of CEA levels in gallbladder bile.