Open AccessUse of Monoclonal Antiestrogen Receptor Antibody to Evaluate Estrogen Receptor Content in Fine Needle Aspiration Breast Biopsies
Author(s) -
Julie L. Flowers,
Gary V. Burton,
Edwin B. Cox,
Kenneth S. McCarty,
Georgette A. Dent,
Geisinger Kr
Publication year - 1986
Publication title -
annals of surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.153
H-Index - 309
eISSN - 1528-1140
pISSN - 0003-4932
DOI - 10.1097/00000658-198603000-00005
Subject(s) - medicine , estrogen receptor , immunohistochemistry , monoclonal antibody , pathology , breast cancer , receptor , mammary gland , fine needle aspiration , antiestrogen , monoclonal , estrogen , progesterone receptor , antibody , biopsy , staining , cancer , immunology
A monoclonal antibody prepared against estrogen receptor has been shown to be highly specific and sensitive for the detection and quantification of estrogen receptor in human breast lesions using immunohistochemical methods. A semiquantitative relationship has been shown between the intensity of staining and biochemical receptor analysis. To evaluate the usefulness of this technique in fine needle aspiration biopsies, 41 cases of breast cancer were studied. Nuclear localization of receptor antibody PAP complex was observed. Comparison of the immunocytochemical analysis of the aspiration cytologic specimens to the biochemical analysis of the excised tumor mass revealed a sensitivity and specificity of 80% and 89%, respectively. There was a significant semiquantitative correlation between the methods. These results extend the observation that antireceptor monoclonal antibody in immunohistochemical analysis is an effective tool in the evaluation of estrogen receptor content in human breast lesions and with careful specimen handling and proper controls may be extended to the evaluation of fine needle aspiration biopsies.