Open AccessProtection by Plasma Proteins of the Isolated Lamb Heart Perfused with Stroma-free Hemoglobin at 38°
Author(s) -
Jacques Suaudeau,
Jonathan Fallon,
E. Kunica,
W. Gerald Austen,
A. John Erdmann
Publication year - 1979
Publication title -
annals of surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.153
H-Index - 309
eISSN - 1528-1140
pISSN - 0003-4932
DOI - 10.1097/00000658-197903000-00013
Subject(s) - extravasation , edema , medicine , interstitial space , hemoglobin , albumin , vascular permeability , endocrinology , stroma , mitochondrion , endothelium , perfusion , swelling , pathology , biochemistry , biology , immunohistochemistry
Isolated lamb hearts were perfused for eight hours at 38 degrees with stroma-free hemoglobin solution (SFHS). The preservation of cardiac structure and function was studied. Control hearts perfused with blood (N = 6) developed no ventricular failure or significant weight gain (13% +/- 5), showed no alteration of cellular ultrastructure, and little interstitial edema. Hearts perfused with 7% (N = 7) or 11% (N = 5) SFHS contracted less well, became edematous (22% and 44% weight gain) and showed altered mitochondria, capillary endothelial swelling and hemoglobin extravasation into the interstitial space. The addition of 5-7% albumin to SFHS (N = 9) markedly reduced interstitial edema (weight gain 11% +/- 13), preserved mitochondria, prevented endothelial swelling, and limited transcapillary escape of hemoglobin. Thus isolated hearts perfused with SFHS develop vascular endothelial damage and an increase in capillary permeability. The addition of plasma proteins to the perfusate protects against this injury.