Open AccessLong-term Patency of Vein Grafts Preserved in Liquid Nitrogen in Dimethyl Sulfoxide
Author(s) -
Thomas Weber,
S. Martin Lindenauer,
T. Dent,
Edward Allen,
Cláudio A Salles,
Lee Weatherbee
Publication year - 1976
Publication title -
annals of surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.153
H-Index - 309
eISSN - 1528-1140
pISSN - 0003-4932
DOI - 10.1097/00000658-197612000-00008
Subject(s) - dimethyl sulfoxide , medicine , cryopreservation , jugular vein , carotid arteries , liquid nitrogen , common carotid artery , surgery , vein , nitrogen , organic chemistry , chemistry , embryo , biology , microbiology and biotechnology
Autogenous canine jugular veins were stored in 15% dimethyl sulfoxide (DMSO) in liquid nitrogen vapor for one to 28 days and then implanted in the carotid artery as autografts. The patency rate at one year was 62.5-87.5%. The patency rate of fresh jugular vein autografts placed in the carotid artery for one year was 75%. Similar autografts stored in liquid nitrogen vapor for one to 28 days without the cryopreservative DMSO exhibited a zero to 12.5% patency rate at one year. Scanning electron microscope studies revealed preservation of theendothelium in DMSO protected veins and a damaged or sloughed endothelium in veins frozen without DMSO cryopreservation.