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A src‐like kinase activates outwardly rectifying chloride channels in CFTR‐defective lymphocytes
Author(s) -
Lepple-Wienhues Albecht,
Wieland Ulrich,
Laun Tilmann,
Heil Luzia,
Stern Martin,
Lang Florian
Publication year - 2001
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fsb2fj000264com
Subject(s) - chloride channel , cystic fibrosis , cystic fibrosis transmembrane conductance regulator , chemistry , intracellular , proto oncogene tyrosine protein kinase src , microbiology and biotechnology , tyrosine kinase , chloride , kinase , biochemistry , biology , signal transduction , medicine , organic chemistry
Defective activation of chloride channels is a hallmark of cystic fibrosis (CF). Recently we have described activation of a volume‐sensitive, outwardly rectifying chloride conductance (I OR ) through the src‐like tyrosine kinase p56 lck . Here we show that p56 lck activates I OR independently of CFTR. In lymphocytes from healthy donors, chloride channels could be opened by either intracellular cAMP, p56 lck or osmotic swelling. In CF lymphocytes, p56 lck and cell swelling but not cAMP could activate chloride channels. Regulation of I OR by p56 lck thus represents an alternative pathway of stimulating membrane chloride conductance that is left intact in cystic fibrosis.—Lepple‐Wienhues, A., Wieland, U., Laun, T., Heil, L., Stern, M., Lang, F. A src‐like kinase activates outwardly rectifying chloride channels in CFTR‐defective lymphocytes. FASEB J. 15, 927–931 (2001)