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The egr‐1 promoter contains information for constitutive and inducible expression in transgenic mice
Author(s) -
Tsai Jo C.,
Liu Lixin,
Cooley Brian C.,
DiChiara Maria R.,
Topper James N.,
Aird William C.
Publication year - 2000
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.99-1072fje
Subject(s) - reporter gene , transgene , gene expression , biology , gene , genetically modified mouse , microbiology and biotechnology , regulation of gene expression , extracellular , endogeny , genetics , endocrinology
Egr‐1 is an immediate early gene that couples short‐term changes in the extracellular milieu to long‐term changes in gene expression. Under in vitro conditions, the Egr‐1 gene is expressed in many cell types and is induced by a wide variety of extracellular signals. The mechanisms by which the Egr‐1 gene is regulated in vivo remain poorly understood. In this study, we have generated transgenic mice with a construct containing 1200 bp of the mouse Egr‐1 promoter coupled to nuclear localized LacZ . In multiple independent lines of mice, reporter gene expression was detected in subsets of endothelial cells, vascular smooth‐muscle cells, cardiomyocytes, neurons, and hepatocytes. This pattern closely resembled that of the endogenous gene. After partial hepatectomy, reporter gene activity was upregulated between two‐ and fivefold in regenerating livers. Taken together, these findings suggest that the Egr‐1 promoter contains information for appropriate spatial and temporal expression in vivo .