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Probiotic Lactiplantibacillus plantarum N‐1 could prevent ethylene glycol‐induced kidney stones by regulating gut microbiota and enhancing intestinal barrier function
Author(s) -
Wei Zhitao,
Cui Yaqian,
Tian Lei,
Liu Yu,
Yu Yang,
Jin Xi,
Li Hong,
Wang Kunjie,
Sun Qun
Publication year - 2021
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.202100887rr
Subject(s) - barrier function , lachnospiraceae , intestinal permeability , gut flora , kidney stones , kidney , chemistry , probiotic , lactulose , medicine , endocrinology , biology , biochemistry , bacteria , microbiology and biotechnology , genetics , 16s ribosomal rna , gene , firmicutes
Defective permeability barrier is considered to be an incentive of hyperuricemia, however, the link between them has not been proven. Here, we evaluated the potential preventive effects of Lactiplantibacillus plantarum N‐1 ( LPN1 ) on gut microbiota and intestinal barrier function in rats with hyperoxaluria‐induced kidney stones. Male rats were supplied with 1% ethylene glycol (EG) dissolved in drinking water for 4 weeks to develop hyperoxaluria, and some of them were administered with LPN1 for 4 weeks before EG treatment as a preventive intervention. We found that EG not only resulted hyperoxaluria and kidney stone formation, but also promoted the intestinal inflammation, elevated intestinal permeability, and gut microbiota disorders. Supplementation of LPN1 inhibited the renal crystalline deposits through reducing urinary oxalic acid and renal osteopontin and CD44 expression and improved EG‐induced intestinal inflammation and barrier function by decreasing the serum LPS and TLR4/NF‐κB signaling and up‐regulating tight junction Claudin‐2 in the colon, as well as increasing the production of short‐chain fatty acid (SCFAs) and the abundance of beneficial SCFAs‐producing bacteria, mainly from the families of Lachnospiraceae and Ruminococcaceae . Probiotic LPN1 could prevent EG‐induced hyperoxaluria by regulating gut microbiota and enhancing intestinal barrier function.