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Starvation induces an increase in intracellular calcium and potentiates the progesterone‐induced mouse sperm acrosome reaction
Author(s) -
SánchezCárdenas Claudia,
Romarowski Ana,
Orta Gerardo,
De la VegaBeltrán José Luis,
MartínHidalgo David,
HernándezCruz Arturo,
Visconti Pablo E.,
Darszon Alberto
Publication year - 2021
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.202100122r
Subject(s) - acrosome reaction , ionophore , sperm , calcium , motility , intracellular , chemistry , starvation , calcium in biology , sperm motility , endocrinology , medicine , microbiology and biotechnology , andrology , biology , biochemistry , organic chemistry
We have recently reported two different methodologies that improve sperm functionality. The first method involved transient exposure to the Ca 2+ ionophore A 23187 , and the second required sperm incubation in the absence of energy nutrients (starvation). Both methods were associated with an initial loss of motility followed by a rescue step involving ionophore removal or addition of energy metabolites, respectively. In this work, we show that starvation is accompanied by an increase in intracellular Ca 2+ ([Ca 2+ ] i ). Additionally, the starved cells acquire a significantly enhanced capacity to undergo a progesterone‐induced acrosome reaction. Electrophysiological measurements show that CatSper channel remains active in starvation conditions. However, the increase in [Ca 2+ ] i was also observed in sperm from CatSper null mice. Upon starvation, addition of energy nutrients reversed the effects on [Ca 2+ ] i and decreased the effect of progesterone on the acrosome reaction to control levels. These data indicate that both methods have common molecular features.