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Sex‐specific effects of in vitro fertilization on adult metabolic outcomes and hepatic transcriptome and proteome in mouse
Author(s) -
Narapareddy Laren,
RhonCalderon Eric A.,
Vrooman Lisa A.,
Baeza Josue,
Nguyen Duy K.,
Mesaros Clementina,
Lan Yemin,
Garcia Benjamin A.,
Schultz Richard M.,
Bartolomei Marisa S.
Publication year - 2021
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.202002744r
Subject(s) - offspring , biology , in vitro fertilisation , transcriptome , pregnancy , metabolic syndrome , placenta , andrology , physiology , endocrinology , medicine , fetus , obesity , gene expression , genetics , gene
Although in vitro fertilization (IVF) is associated with adverse perinatal outcomes, there is increasing concern about the long‐term and sex‐specific health implications. Augmenting our IVF mouse model to longitudinally investigate metabolic outcomes in offspring from optimal neonatal litter sizes, we found sex‐specific metabolic outcomes in IVF offspring. IVF‐conceived females had higher body weight and cholesterol levels compared to naturally conceived females, whereas IVF‐conceived males had higher levels of triglycerides and insulin, and increased body fat composition. Through adult liver transcriptomics and proteomics, we identified sexually dimorphic dysregulation of the sterol regulatory element‐binding protein (SREBP) pathways that are associated with the sex‐specific phenotypes. We also found that global loss of DNA methylation in placenta was linked to higher cholesterol levels in IVF‐conceived females. Our findings indicate that IVF procedures have long‐lasting sex‐specific effects on metabolic health of offspring and lay the foundation to utilize the placenta as a predictor of long‐term outcomes.