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hnRNP K supports the maintenance of RORγ circadian rhythm through ERK signaling
Author(s) -
Kwon Paul Kwangho,
Kim HyoMin,
Kang Byunghee,
Kim Sung Wook,
Hwang SungMin,
Im SinHyeog,
Roh TaeYoung,
Kim KyongTai
Publication year - 2021
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.202002076r
Subject(s) - circadian rhythm , rhythm , mapk/erk pathway , biology , microbiology and biotechnology , medicine , endocrinology , signal transduction
Retinoic acid‐related orphan receptor γ ( RORγ ) maintains the circadian rhythms of its downstream genes. However, the mechanism behind the transcriptional activation of RORγ itself remains unclear. Here, we demonstrate that transcription of RORγ is activated by heterogeneous nuclear ribonucleoprotein K (hnRNP K) via the poly(C) motif within its proximal promoter. Interestingly, we confirmed the binding of endogenous hnRNP K within RORγ1 and RORγ2 promoter along with the recruitment of RNA polymerase 2 through chromatin immunoprecipitation (ChIP). Furthermore, an assay for transposase accessible chromatin (ATAC)‐qPCR showed that hnRNP K induced higher chromatin accessibility within the RORγ1 and RORγ2 promoter. Then we found that the knockdown of hnRNP K lowers RORγ mRNA oscillation amplitude in both RORγ and RORγ ‐dependent metabolic genes. Moreover, we demonstrated that time‐dependent extracellular signal‐regulated kinase (ERK) activation controls mRNA oscillation of RORγ and RORγ ‐dependent metabolic genes through hnRNP K. Taken together, our results provide new insight into the regulation of RORγ by hnRNP K as a transcriptional activator, along with its physiological significance in metabolism.