Premium
CircARID1A regulates mouse skeletal muscle regeneration by functioning as a sponge of miR‐6368
Author(s) -
Liu Jia,
Li MengLu,
Kong LingHao,
Cao MengWen,
Zhang MoLan,
Wang YanHong,
Song ChengChuang,
Fang XingTang,
Chen Hong,
Zhang ChunLei
Publication year - 2021
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.202001992r
Subject(s) - regeneration (biology) , sponge , skeletal muscle , microbiology and biotechnology , chemistry , anatomy , biology , paleontology
The noncoding RNAs play important role in growth and development of mammalian skeletal muscle. Recent work has shown that circRNAs are abundant in skeletal muscle tissue, with significant changes in their expression patterns during muscle development and aging. We identified a novel circRNA called circARID1A that is highly expressed in mice skeletal muscle compare to its linear transcript. Experiments shown that circARID1A significantly inhibited the process of C2C12 cell proliferation and promoted its differentiation. Interactions between circRNA and miRNA were screened by miRNA gene chip sequencing. The results indicated that circARID1A can sponge miR‐6368, which was further verified by miRNA sensor and other experiments. Besides, miR‐6368 is a commonly expressed miRNA that regulates the expression of several target genes including Tlr4. A mouse model of skeletal muscle injury was successfully established to explore the role of circARID1A in skeletal muscle development and regeneration in vivo. Moreover, we found the overexpression of circARID1A significantly promoted the regeneration of skeletal muscle. The results of our study suggest that circARID1A may regulate skeletal muscle cell development and regeneration by sponging miR‐6368.