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Single‐cell RNA sequencing reveals the induction of novel myeloid and myeloid‐associated cell populations in visceral fat with long‐term obesity
Author(s) -
Harasymowicz Natalia S.,
Rashidi Neda,
Savadipour Alireza,
Wu ChiaLung,
Tang Ruhang,
Bramley John,
Buchser William,
Guilak Farshid
Publication year - 2021
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.202001970r
Subject(s) - st louis , medicine , gerontology , art history , history
Macrophages and other immune cells are important contributors to obesity‐associated inflammation; however, the cellular identities of these specific populations remain unknown. In this study, we identified individual populations of myeloid cells found in mouse epididymal/visceral adipose tissue by single‐cell RNA sequencing, immunofluorescence, and flow cytometry. Multiple canonical correlation analysis identified 11 unique myeloid and myeloid‐associate cell populations. In obese mice, we detected an increased percentage of monocyte‐derived pro‐inflammatory cells expressing Cd9 and Trem2 , as well as significantly decreased percentages of multiple cell populations, including tissue‐resident cells expressing Lyve1 , Mafb , and Mrc1 . We have identified and validated a novel myeloid/macrophage population defined by Ly6a expression, exhibiting both myeloid and mesenchymal characteristics, which increased with obesity and showed high pro‐fibrotic characteristics in vitro. Our mouse adipose tissue myeloid cell atlas provides an important resource to investigate obesity‐associated inflammation and fibrosis.