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Lysyl oxidase interactions with transforming growth factor‐β during angiogenesis are mediated by endothelin 1
Author(s) -
Grunwald Hagar,
Hunker Kristina L.,
Birt Isabelle,
Lulu Chen,
Aviram Rohtem,
ZaffryarEilot Shelly,
Ganesh Santhi K.,
Hasson Peleg
Publication year - 2021
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.202001860rr
Subject(s) - lysyl oxidase , crosstalk , angiogenesis , microbiology and biotechnology , transforming growth factor , vascular endothelial growth factor , biology , growth factor , endothelin 1 , vascular smooth muscle , signal transduction , transforming growth factor beta , chemistry , extracellular matrix , vegf receptors , cancer research , endocrinology , genetics , smooth muscle , receptor , physics , optics
Crosstalk between multiple components underlies the formation of mature vessels. Although the players involved in angiogenesis have been identified, mechanisms underlying the crosstalk between them are still unclear. Using the ex vivo aortic ring assay, we set out to dissect the interactions between two key angiogenic signaling pathways, vascular endothelial growth factor (VEGF) and transforming growth factor β (TGFβ), with members of the lysyl oxidase (LOX) family of matrix modifying enzymes. We find an interplay between VEGF, TGFβ, and the LOXs is essential for the formation of mature vascular smooth muscle cells (vSMC)‐coated vessels. RNA sequencing analysis further identified an interaction with the endothelin‐1 pathway. Our work implicates endothelin‐1 downstream of TGFβ in vascular maturation and demonstrate the complexity of processes involved in generating vSMC‐coated vessels.