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Exosomal miR‐145‐5p derived from orthohantavirus‐infected endothelial cells inhibits HTNV infection
Author(s) -
Wang Xin,
Chen QingZhou,
Zan YuXing,
Wang MeiRong,
Yan Jie,
Guo WeiWei,
Li KaiJi,
Liu YuanYuan,
Luo Fan,
Feng Yong,
Hou Wei,
Xiong HaiRong
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.202001114r
Subject(s) - biology , virology , chemistry
Human infection of orthohantavirus can cause potentially fatal diseases, such as hemorrhagic fever with renal syndrome (HFRS) caused by Hantaan virus (HTNV) in Eurasia. Exosomes are new carriers for information exchange between cells. Cumulative findings suggest that exosomes released from parental infected cells can block or promote viral infection in recipient cells, but the role of exosomes in hantavirus infection is poorly understood. In our study, we identified the exosomes derived from HTNV‐infected human vascular endothelial cells (HUVECs) (Exo‐HV) and found the antiviral properties of Exo‐HV in the uninfected recipient cells. High‐throughput sequencing revealed the distinctly expressed miRNAs transcriptomes in Exo‐HV. MiR‐145‐5p, one of the abundant miRNAs packaged into Exo‐HV, was found to be able to transferred to recipient cells and functioned by directly targeting M RNA of HTNV 76‐118 and inducing type I interferon (IFN‐I) response, thus, blocking the viral replication. Concluding, this study indicated that exosomes released by HTNV‐infected HUVECs were able to transfer active molecules, miR‐145‐5p as a proving sample, to mediate novel anti‐HTNV activity in the neighboring uninfected cells, which will help us to explore new strategies for the treatment of infectious disease utilizing exosomes with miRNA.