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The relationship between gut microbiota and short chain fatty acids in the renal calcium oxalate stones disease
Author(s) -
Liu Yu,
Jin Xi,
Hong Hyokyoung G.,
Xiang Liyuan,
Jiang Qingyao,
Ma Yucheng,
Chen Zude,
Cheng Liang,
Jian Zhongyu,
Wei Zhitao,
Ai Jianzhong,
Qi Shiqian,
Sun Qun,
Li Hong,
Li Yi,
Wang Kunjie
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.202000786r
Subject(s) - oxalate , calcium oxalate , calcium , feces , bacteria , kidney stones , microbiology and biotechnology , gut flora , urinary system , medicine , chemistry , biochemistry , food science , biology , inorganic chemistry , genetics
Abstract The relationship of gut microbiota and calcium oxalate stone has been limited investigated, especially with no study of gut microbiota and short chain fatty acids (SCFAs) in nephrolithiasis. We provided Sprague Dawley rats of renal calcium oxalate stones with antibiotics and examined the renal crystals deposition. We also performed a case‐control study by analyzing 16S rRNA microbial profiling, shotgun metagenomics and SCFAs in 153 fecal samples from non‐kidney stone (NS) controls, patients with occasional renal calcium oxalate stones (OS) and patients with recurrent stones (RS). Antibiotics reduced bacterial load in feces and could promote the formation of renal calcium crystals in model rats. In addition, both OS and RS patients exhibited higher fecal microbial diversity than NS controls. Several SCFAs‐producing gut bacteria, as well as metabolic pathways associated with SCFAs production, were considerably lower in the gut microbiota among the kidney stone patients compared with the NS controls. Representation of genes involved in oxalate degradation showed no significance difference among groups. However, fecal acetic acid concentration was the highest in RS patients with high level of urinary oxalate, which was positively correlated with genes involvement in oxalate synthesis. Administration of SCFAs reduced renal crystals. These results shed new light on bacteria and SCFAs, which may promote the development of treatment strategy in nephrolithiasis.