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Colchicine treatment impairs skeletal muscle mitochondrial function and insulin sensitivity in an age‐specific manner
Author(s) -
Buch Bjørg Thiellesen,
Halling Jens Frey,
Ringholm Stine,
Gudiksen Anders,
Kjøbsted Rasmus,
Olsen Mette Algot,
Wojtaszewski Jørgen F. P.,
Pilegaard Henriette
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201903113rr
Subject(s) - skeletal muscle , medicine , endocrinology , autophagy , soleus muscle , sarcopenia , glucose homeostasis , biology , insulin resistance , basal (medicine) , colchicine , mitochondrion , glucose uptake , insulin , chemistry , microbiology and biotechnology , biochemistry , apoptosis
Abstract The aim of the study was to investigate the impact of autophagy inhibition on skeletal muscle mitochondrial function and glucose homeostasis in young and aged mice. The transcriptional co‐activator PGC‐1α regulates muscle oxidative phenotype which has been shown to be linked with basal autophagic capacity. Therefore, young and aged inducible muscle‐specific PGC‐1α knockout (iMKO) mice and littermate lox/lox controls were used in three separate experiments performed after either saline or colchicine injections on two consecutive days: (1) Euthanization in the basal state obtaining skeletal muscle for mitochondrial respirometry, (2) whole body glucose tolerance test, and (3) in vivo insulin‐stimulated 2‐deoxyglucose (2‐DG) uptake into skeletal muscle. Muscle PGC‐1α was not required for maintaining basal autophagy flux, regardless of age. Colchicine‐induced inhibition of autophagy was associated with impairments of skeletal muscle mitochondrial function, including reduced ADP sensitivity and altered mitochondrial redox balance in both young and aged mice. Colchicine treatment reduced the glucose tolerance in aged, but not young mice, and similarly in iMKO and lox/lox mice. Colchicine reduced insulin‐stimulated 2‐DG uptake in soleus muscle in aged mice, independently of PGC‐1α, and without affecting insulin‐regulated phosphorylation of proximal or distal mediators of insulin signaling. In conclusion, the results indicate that autophagy regulates the mitochondrial ADP sensitivity and redox balance as well as whole body glucose tolerance and skeletal muscle insulin sensitivity in aged mice, with no additional effects of inducible PGC‐1α deletion.