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Peroxidasin is essential for endothelial cell survival and growth signaling by sulfilimine crosslink‐dependent matrix assembly
Author(s) -
Lee SeungWoo,
Kim HyunKyung,
Naidansuren Purevjargal,
Ham Kyung A,
Choi Hong Seok,
Ahn HyunYoung,
Kim Myungshin,
Kang Dong Hoon,
Kang Sang Won,
Joe Young Ae
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201902899r
Subject(s) - microbiology and biotechnology , fibronectin , extracellular matrix , cell growth , transfection , laminin , chemistry , small interfering rna , cell migration , cell , biology , biochemistry , gene
Abstract Peroxidasin (PXDN) has been reported to crosslink the C‐terminal non‐collagenous domains of collagen IV (Col IV) by forming covalent sulfilimine bond. Here, we explored the physiological role of PXDN and its mechanism of action in endothelial cell survival and growth. Silencing of PXDN using siRNAs decreased cell proliferation without increase of the number of detached cells and decreased cell viability under serum‐starved condition with increased fragmented nuclei and caspase 3/7 activity. Conditioned medium (CM) containing wild‐type PXDN restored the proliferation of PXDN‐depleted cells, but CM containing mutant PXDN with deletion of either N‐terminal extracellular matrix (ECM) motifs or peroxidase domain failed to restore PXDN function. Accordingly, anti‐PXDN antibody [raised against IgC2 (3‐4) subdomain within ECM motifs] and peroxidase inhibitor phloroglucinol prevented the rescue of the PXDN‐depleted cells by PXDN‐containing CM. PXDN depletion resulted in loss of sulfilimine crosslinks, and decreased dense fibrillar network assembly of not only Col IV, but also fibronectin and laminin like in Col IV knockdown. Exogenous PXDN‐containing CM restored ECM assembly as well as proliferation of PXDN‐depleted cells. Accordingly, purified recombinant PXDN protein restored the proliferation and ECM assembly, and prevented cell death of the PXDN‐depleted cells. PXDN depletion also showed reduced growth factors‐induced phosphorylation of FAK and ERK1/2. In addition, siPXDN‐transfected cell‐derived matrix failed to provide full ECM‐mediated activation of FAK and ERK1/2. These results indicate that both the ECM motifs and peroxidase activity are essential for the cellular function of PXDN and that PXDN is crucial for ECM assembly for survival and growth signaling.